Figure 5
miR-144 suppressed glioma cells proliferation through targeting FGF7 and inhibiting downstream Akt signaling. (a) The overexpression efficiency of CAV2 and FGF7 was identified after lentivirus infection. (b) U251 cells were transfected with miR-144 mimic and regained expression of FGF7 or CAV2. The cell proliferation was detected (n = 4). (c) Specific siRNAs for FGF7 or CAV2 were designed and the efficiency was detected (n = 4). (d) U251 cells were transfected with miR-144 inhibitor followed inhibition of FGF7 or CAV2. The cell proliferation was detected (n = 4). (e) U251 cells were treated as same as (b) the cell cycle was analysis (n = 4). (f) U251 cells were treated as same as (d) the cell cycle was analysis (n = 4). (g,h) U251 cells were treated as same as (e,f), and protein levels of Akt and phos-Akt were determined (n = 4). (i) U251 cells were treated as same as (d), and ROS production was evaluated (n = 4). Bars represent means ± SD, *P < 0.05, **P < 0.01, ***P < 0.001.
