Figure 3
From: Glycation changes molecular organization and charge distribution in type I collagen fibrils
(A,B) Video camera images of a region of the HOPG sample with a collagen fibril before (A) and after (B) R5P treatment. (C,D) Representative AFM topography height images of collagen fibril number 2 from (A,B). Unglycated (C) and R5P-glycated (D) fibril section. (E,F) show longitudinal height profiles of dashed lines in (C,D), respectively. (G,H) Representative KFM surface potential maps of collagen fibril number 2 from (A,B). Unglycated (G) and R5P-glycated (H) fibril section. (J,K) show longitudinal potential profiles of dashed lines in (G,H), respectively. Surface potential values are with respect to surrounding HOPG as base level. Images and profiles (C,E,G,J) are on identical ___location of fibril. Images and profiles (D,F,H,K) are on identical ___location of fibril but different ___location from (C,E,G,J). (L) Effect of glycation on surface potential of overlap and gap zones of individually identified collagen fibrils (N = 11) measured by KFM. For each fibril, the overlap and gap zone surface potential reduction after R5P incubation is shown.
