Table 1 LC-MS analysis of enzymatic cross links and pre-existing glucose glycation for type I collagen used in this work and ribose-5-phosphate glycated collagen per mole of collagen (mole/ mole(Pro + Hyp)/669); standard deviations in brackets.

From: Glycation changes molecular organization and charge distribution in type I collagen fibrils

sample

HLNL

DHLNL

LNL

PYD

DPD

Pre-existing glucose-glycated Lys

Pre-existing glucose-glycated Hyl

Unglycated

1.06 (0.07)

0.140 (0.002)

0.025 (0.002)

0.65 (0.04)

—

0.47 (0.02)

0.15 (0.01)

R5P (50 mM) glycated

0.4 (0.1)

0.09 (0.07)

0.07 (0.04)

0.38 (0.15)

0.11 (0.04)

0.17 (0.05)

0.11 (0.02)

  1. DPD levels in unglycated collagen were too low for measurement. (HLNL = hydroxylysinonorleucine; DHLNL = dihydroxylysinonorleucine; LNL = lysinonorleucine; PYD = pyridinoline; DPD = deoxypyridinoline).
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