Figure 7
A Predicted binding sites of chi-miR-199a-5p and TGF-β2-3′UTR. BC psiCHECK-2 vector structure map and ___location of TGF-β2-3′UTR were cloned into psiCHECK-2 double luciferase reporter gene vector. The gene sequence was inserted into the XhoI and NotI digestion sites, and the expression was regulated by SV40 promoter. The vector contained Luc marker gene expression. D Chi-miR-199a-5p and TGF-β2-3′UTR target site binding sites and TGF-β2 mutation sites. E TGF-β2-3′UTR wild type sequencing results. Grey is shown to be a sequence of clone targets, and yellow shows a predicted binding site. F TGF-β2-3 ‘UTR mutant sequencing results. Grey is shown to be a sequence of clone targets, and yellow shows a predicted binding site. G Detection of chi-miR-199a-5p and TGF-β2-3 ‘UTR interaction by dual luciferase reporter gene.
