Figure 3
From: Complex approach for analysis of snake venom α-neurotoxins binding to HAP, the high-affinity peptide
Rational design of the peptides capable to bind of αCtx. (A) Amino acid sequence alignment of HAP and its point mutant variants: residues numbering is shown above the sequences. Point mutations are underlined. (B) [125I]-αBgt specific binding to T. californica nAChR in the presence of 10 μM HAP and its analogues. (C) [125I]-αCtx specific binding to T. californica nAChR in the presence of 30 μM HAP and its analogues. Total binding is measured in the absence of any peptide and expressed in terms of specific binding (% of the general binding minus non-specific binding).
