Figure 5
From: Complex approach for analysis of snake venom α-neurotoxins binding to HAP, the high-affinity peptide
Fluorescence spectra and fitting results for αBgt-HAP, αBgt-HAP[L9E], αCtx-HAP, αCtx-HAP[L9E] toxin-peptide pairs. (A) Normalized fluorescence spectra of toxin (dark red), peptide (black) and their mixtures with different concentration ratios (dark red to blue). (B) Spectra projections on the first three principal components (dots) and the fit obtained using 1:1 complexation model (solid lines). The nonlinear dependence of the first projection on peptide concentration demonstrates complexation in systems, and the 1:1 model is in a good agreement with the projections obtained from the experimental data. (C) Dependence of partial concentrations of peptide, toxin and complex on total peptide concentrations obtained from PCA projections.
