Figure 3
From: Biochemical features of the novel Tail Tubular Protein A of Yersinia phage phiYeO3-12
Analysis of TTPAgp11 from Yersinia phage phiYeO3-12 using 10% (A) and 12.5% (B) SDS-PAGE. (A) The lanes are as follows: (1) Spectra Multicolor Broad Range Protein Ladder (Bio-Rad); (2) crude extract; (3) pooled fractions after the first round of Ni2+ -affinity chromatography; (4) protein solution after TEV protease cleavage. (B) The lanes are as follows: (1) Color Prestained Protein Standard, Broad Range (Bio-Rad); (2) pure target protein - TTPAgp11. The original pictures of the gels are attached in a Supplementary Data File.
