Figure 3
Expression of mutant p53 mRNA and protein in the brain of WT and p53 mutant mice. (a) p53 mRNA in astrocytoma in situ in p53 mutant mouse. DIG-labelled cRNA of p53 displayed the p53 mRNA expression in both WT and p53 mutant mice bearing astrocytoma in the brain. (b) Mutant p53 protein in the brain of p53 mutant mouse. Mutant p53 protein was detected in the brain and brain tumor of p53 mutant mice, whereas the expression of mutant p53 was not confirmed in the brain of WT mice by immunohistochemistry using antibody against the mutant p53 protein. The enlarged parts of the dotted rectangle appear on the right panel.
