Figure 6

KFD and PNF had no effect on the cognitive function of rats. Rats at 5 weeks of age underwent the first MWM test (Days 1–5), and then, they were administered the drugs (Day 16). For KFD + PNF treatment, the rats received PNF administration once they lost the righting reflex after KFD injection. A month later, the second round of the MWM test (Days 46–50) was performed. The number of attempts to across the platform (A) and the ratio of time spent in the range around the platform (B) of the second round MWM were similar between the control and KFD or KFD + PNF groups. (A,B) The blue individual data represent female rats, and the red data represent male rats. There were no differences in the latency to find the platform (C) or swim distance (D) in the three groups. (E) Representative traces of the movement of the rats in the spatial probe test (Day 50). The blue circle represents the removed platform, and the orange circle represents the range around the platform. Significant differences were determined by two-way analysis of variance, mean ± SD. CON: saline control group, n = 10; KFD, n = 11; KFD + PNF, n = 8.