Figure 3
From: Fluorescence microscopy tensor imaging representations for large-scale dataset analysis
Content visualization and vascular tractography. All representations and visualizations refer to the representative volume of lectin-TRITC stained heart shown in Fig. 2(a). The 3D perspective (a), axial (b) and sagittal (c) vector field representations of the primary eigenvector clearly show the spatial preferential orientation of the microvascular network present in the tissue volume of Fig. 2a. Vectors are color coded to represent orientation with respect to the XYZ lab frame. (d) Map of the principal eigenvector along a sagittal plane (Supplementary Fig. S7g–h) of the volume in (a). The intensity corresponds to the principal eigenvector’s length, with hedgehogs representing its direction in space at each point in the field. (e) Ellipsoid glyph representation of the tensor field for the same sagittal plane. Different streamline groups represent different trajectories along the principal eigenvector direction. (f) Color-coded fiber tracts represent seeded streamlines along the principal eigenvector and illustrate the main distribution and direction of the microvasculature. 3D perspective (g), axial (h) and sagittal (i) vascular tractograms with an associated axial ellipsoid glyph representation. (j–l) Tomographic vascular tractograms obtained along the principal (j), secondary (k) and tertiary (l) eigenvector, respectively. 3D rendering of the vascular skeleton (m) and the segments connecting the graph nodes. Color coded, RGB. Bounding box, 250 × 205 × 340 μm. Scale bars, 50 μm.
