Figure 1 | Scientific Reports

Figure 1

From: Streamlined selection of cancer antigens for vaccine development through integrative multi-omics and high-content cell imaging

Figure 1

Scheme of the rapid high-throughput approach for discovering natural CTL epitopes. Preselected TIL-resident TNBC tumors underwent HLA-peptidomic analysis to identify HLA-bound peptides. Integrated WTS data revealed a higher priority to select promising HLA-peptides via high-resolution bioinformatics analysis, showing immune-cell-specific signatures and TCR-repertoire diversity in tumors. Combined NGS analysis and the use of predictive algorithms for MHC-binding affinity enabled selection of highly immunogenic HLA-peptide candidates. Analysis of IFNγ-producing CD8+ T cell response using the high-content imaging system in a 384-well format at the single-cell level for discovery of immunogenic HLA epitopes eliciting a CTL response.

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