Figure 4
HUVECs tube formation in conditioned media. Number of network branches (a,c) and total tube length (b,d) in HUVECs exposed to conditioned media in normal (a,b) and extended (c,d) treatments: negative control (Starving), positive control (VEGF), singular T3M4, singular NHDF, T3M4-NHDF co-culture (CClt), co-cultured T3M4 (CClt T3M4), co-cultured NHDF (CClt NHDF). All measurements are normalized to the distance between control means. Experiments were performed in triplicate and analysed with ANOVA, using Tukey’s test for pair-wise comparisons: *p < 0.05; **p < 0.01; ***p < 0.001.
