Figure 1
From: Establishment of macaque trophoblast stem cell lines derived from cynomolgus monkey blastocysts
Establishment of cynomolgus monkey trophoblast stem cell lines (macTSC) from blastocysts. (A) Schematic representation of the cell culture conditions and experimental procedure. Blastocysts #1-3 were initially co-cultured with mouse embryonic fibroblasts (MEF) that was substituted by 50 or 70% MEF-conditioned medium (50CM, 70CM) as indicated after the first passage. Abbreviations of the exogenously added reagents are shown. The other two blastocysts (#4 and #5) were cultured without MEF in the TS medium supplemented with FHBY. The fat arrow-like shapes depict each passage. (B) The appearance of the obtained cell line macTSC#2. Scale bar = 100 µm. (C) Growth of macTSC#2 cells over 132 days (from passage number 6 to 39) of culture in the AFHBY condition. At each passage, cells were harvested, counted, and seeded at 1 × 105 per 35-mm dish in triplicate. A mean value of triplicate was used to calculate the theoretical cumulative number. (D) Growth of macTSC#2 cells in different culture conditions. The number of cells normalized to that at day 0, and arbitrarily set as 1. The mean values (±SD) of triplicates of biological duplicates are shown. Statistical analysis was done by the Tukey-Kramer test.
