Figure 4

Gene expression profiling of macTSC lines. (A) Heatmap was drawn by the values of correlation coefficients using the RNA-seq datasets of macTSCs, ESC, fibroblast, and cynomolgus monkey placenta. (B) Enriched GO terms associated with the genes with >2-fold expression in all macTSCs compare to that in ESC. PANTHER (www.pantherdb.org) was used for GO analyses. Gene ratio, the ratio of the analyzed genes in the gene set of the GO term; Count, the number of analyzed genes in the GO term. P-values were calculated using Fisher's exact test with Bonferroni correction. (C,D,E) qRT-PCR for analyzing the expression of CTB (C), STB (D) and EVT (E) markers. The mean values (±SD) of technical replicates for biological duplicates, normalized by the expression of ACTB, were shown relative to those of cells maintained under the FHBY condition (arbitrarily set as 1). −dbcAMP, basal TS medium without FHBY and dbcAMP; +dbcAMP, basal TS medium with 1 mM dbcAMP. *p < 0.05 vs. FHBY (Two-tailed Student's t-test).