Figure 1 | Scientific Reports

Figure 1

From: The Pivotal Roles of US3 Protein in Cell-to-Cell Spread and Virion Nuclear Egress of Duck Plague Virus

Figure 1

Schematic diagram of pBAC-CHv-ΔUS3 construction. (I) The DPV CHv genome consists of the UL, IRS, US and TRS regions. (II) The US3 gene is replaced with the KanR cassette in the first Red recombination through the 40-bp homology arms (sequences a,b). (III) The intermediate product after the first Red recombination is shown. (IV) The KanR cassette is removed in the second Red recombination through I-SceI and a 40-bp sequence duplication (sequence a). (V) The genome of pBAC-CHv-ΔUS3 is shown.

Back to article page