Figure 5

Co-staining of PH3 with Br, Hnt and Cut. (A–C) Confocal images showed the co-stainings, Br, Hnt and Cut antibody staining in green, PH3 in red and DAPI in blue. Co-expressing follicle cells were indicated by arrowheads. (D) Statistical analysis of data obtained from egg chamber staging of transitional Br, Hnt, and Cut expression with PH3 co-staining. Percentage of PH3-positive egg chambers were labeled on top of each column. Scale bar =10 µm.