Figure 3
The early skin IFN response to UV light exposure is cGAS dependent. Age-matched female B6 (wild type, WT), cGAS−/−, and Ifnar1−/− mice were exposed to a single dose of UVB light as in Fig. 2. Skin biopsies were obtained prior to UVB light exposure and at 6 and 24 h after irradiation. (A) Fold change in the expression of IFN-I stimulated genes (ISG) in the skin was determined relative to baseline, i.e. non-irradiated skin (no UV). (B) Skin IFN scores at 6 and 24 hours after UVB were calculated as sum of normalized expression levels of the same 7 ISG. (C) Fold-induction in the expression of inflammatory cytokines Tnfa, Il-6, and Il1-β were determined relative to baseline, i.e. non-irradiated skin (no UV). Statistical significance was determined by Student’s t-test (n = 4–8; *p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant).
