Figure 4
From: miR-379 links glucocorticoid treatment with mitochondrial response in Duchenne muscular dystrophy
Myogenic effect of inhibition of EIF4G2 and DAPIT. (a,b) Knocking down of EIF4G2 and DAPIT expression delays differentiation of AB1190 myoblast. AB1190 myoblast were treated by shRNA (shScramble, shEIF4G2, shDAPIT). Cells were differentiated for 4 days. Myotubes containing 6 or more nuclei (yellow arrows in representative images in 3 h) were counted (presented graphically in b). (c,d) Muscle regulatory factors (MRF) expression in AB1190 myoblast with knocked-down expression by shRNA of EIF4G2 (c) and DAPIT (d). (e,f) Transcriptional expression of genes associated with myogenic differentiation in shRNA knock-down EIF4G2 (e) and DAPIT (f).
