Figure 5
From: miR-379 links glucocorticoid treatment with mitochondrial response in Duchenne muscular dystrophy
DAPIT IHC localization, mitochondrial functional studies in M180 iPS cells. (a) Co-localization of DAPIT with ATP5a (mitochondrial complex 5) into oxidative fibers (NADH positive) in transversal section of quadriceps muscle from adult C57Bl/10 mouse. (b) Co-localization DAPIT and ATP5a in single fibers isolated from the gastrocnemius of adult C57Bl/10 mouse. (c,d) EIF4G2 and DAPIT mRNA (c) and protein expressions (d) in EIF4G2 and DAPIT knocked-down M180 iPS-derived skeletal myoblasts (skMPC). (e,f,g) Mitochondrial complex 5 to 1 activity ratio (e) and absolute activity (f,g) in EIF4G2 and DAPIT knocked-down skMPC. (h,i) TFAM (h) and PGC-1α (i) expression in knocked-down skMPC. (j) ATP to ADP ration in DAPIT knockdown (sh-DAPIT) versus control (sh-Scramble) and untreated AB1190 (n = 4, in 3 independent experiments) in vitro differentiated myotubes.
