Figure 3 | Scientific Reports

Figure 3

From: Antitumor effect of a WEE1 inhibitor and potentiation of olaparib sensitivity by DNA damage response modulation in triple-negative breast cancer

Figure 3

WEE1 inhibition resulted in HR impairment. (a) The expression levels of DNA damage response proteins were assessed by western blotting after treatment of AZD1775 350 nmol/L with MDA-MB-231, BT-549 and MDA-MB-468 for 48 h. (b) AZD1775 caused DNA damage accumulation. Cells were treated with 350 nmol/L AZD1775 for 5 d and DNA double-strand breaks in cells were assessed using a comet assay. Data presented are the mean tail length ±SD from a single representative experiment (n = 3). The scale bar indicates 200 µm. *P < 0.05, **P < 0.01. (c) AZD1775 inhibited the formation of RAD51 foci at sites of DNA damage. After treatment with 350 nmol/L of AZD1775 for 48 h, numbers of RAD51 (red) and γ-H2AX (green) foci in cells were determined by immunoblotting and confocal microscopy. A total of 100 cells per group were analyzed, and cells with at least 5 foci were regarded as positive. DNA was counterstained with DAPI (blue). The scale bar indicates 10 µm. Bars represent ±SDs (n = 3). **P < 0.01. We used several gels to examine protein expression. But the results were all derived the same experiment, and the gels and blots were processed in parallel.

Back to article page