Figure 1
From: WNT and inflammatory signaling distinguish human Fallopian tube epithelial cell populations
Bulk Fallopian tube organoid formation assays. (A) Schematic of the proximal and distal regions of Fallopian tubes. The frequency (B) and the diameter (C) of the distal and proximal TE orgnoids were measured on the 8th day in the culture successively for 4 passages. Each dot corresponds to an observation. (D) Representative images of human distal and proximal tube epithelial organoids. First row shows the phase contrast (PhC) images of the organoids. The expression of ACTUB (second row), PAX8 (third row), and ALDH1A1 (fourth row) in distal and proximal tubal epithelial organoids. Note the cilia are marked by ACTUB (green), nuclear staining (green) for PAX8 and cytoplasmic staining (green) for ALDH1A1. Counterstaining with DAPI for immunofluorescence staining. Bar, D, First row, 150 μm; Second row, 60 μm; third row, 50 μm; and fourth row, 50 μm.
