Figure 8 | Scientific Reports

Figure 8

From: Tetanus insensitive VAMP2 differentially restores synaptic and dense core vesicle fusion in tetanus neurotoxin treated neurons

Figure 8

VAMP1 is not required for DCV fusion. (A) Panels show a neurite before stimulation (Baseline), during stimulation displaying fusion events (Stimulation), and the total number of NPY-pHluorin labeled DCVs upon NH4+ perfusion (Total DCV pool). Scale bar 5 µm. (B) Total number of NPY-pHluorin (infected at DIV 9) labeled DCVs (imaged at DIV 14) in control (n = 16, N = 3) and vamp1–/– (n = 19, N = 3) neurons. Unpaired Student's t-test: p = 0.18 (ns). (C, D) Histograms of DCV fusion events in control (C) and vamp1–/– (D) hippocampal neurons. (E) Cumulative DCV fusion events in control and vamp1–/– hippocampal neurons. Shaded area represents SEM. Blue bars indicate 16 trains of 50 AP at 50 Hz interspaced by 0.5 s. (F) Average DCV fusion events per cell for control (n = 16, N = 3) and vamp1–/– (n = 19, N = 3) neurons. Unpaired Student's t-test: p = 0.35 non-significant (ns). Bars represent mean + SEM. Detailed statistics are shown in Supplementary Table S1.

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