Figure 2

Biofilm formation of Bifidobacterium breve UCC2003 in response to bile salts. Biofilm formation was induced by addition of glycocholic acid (GC), taurocholic acid (TC), chenodeoxycholic acid (CDC), taurodeoxycholic acid (TDC) and glycodeoxycholic acid (GDC) at concentrations of 1 mM, 10 mM, 20 mM and 40 mM. Biofilm was allowed to form for 24 h, was staining using crystal violet and the absorbance read at O.D.570 nm. Experiments were carried out in triplicate and error bars represent standard error of the mean.