Figure 1

(A) A total of 40 mice were subcutaneously inoculated with H460 and H460/MX20 cells (≈ 5 × 106) in the right flank, respectively. (B, C) The changes in tumor volume and body weight over time following the implantation. Data points represented the mean ± SD of tumor volumes and body weight from each group. n = 20. (D) Solid tumor formation rate of H460 and H460/MX20 cells (100%). (E) The selected cell xenografts were cut into about 5 mm × 5 mm and fixed with 10% neutral formalin. (F) ABCG2 expression analysis by immunohistochemistry in tumor tissues collected from H460 and H460/MX20 cell xenografts.