Figure 2
From: Zonisamide ameliorates progression of cervical spondylotic myelopathy in a rat model
Temporal profiles of motor deficits of CSM rats with or without Zonisamide (ZNS) administration. (A) Experimental protocol of gait and histological analyses of CSM rats. Water-absorbing polyurethane blade was implanted at 12 weeks of age. Operated rats were divided into three groups: sham-operated [sham; n = 6 rats], daily intragastric administration of 30 mg/kg ZNS in 0.5% methylcellulose [ZNS( +); n = 11 rats] and 0.5% methylcellulose alone as a control [ZNS(-); n = 11 rats]. (B–E) Stride length (B and C) and the duty cycle (D and E) of fore- and hind-limbs were analyzed with CatWalk. At 10 weeks after surgery, ZNS increased the stride length of forelimbs (B) and hindlimbs (C), and reduced the duty cycle of hindlimbs (E). * p < 0.05 and ** p < 0.01 by one-way ANOVA followed by Tukey HSD. (F) Ratio of slipped steps on a ladder bar was analyzed by the wire mesh walking test. Mean and SD are indicated (n = 12 limbs in 6, 22 limbs in 11, and 22 limbs in 11 for sham-operated, ZNS(-) and ZNS( +) rats, respectively). * p < 0.05 and *** p < 0.001 by two-way repeated measured ANOVA followed by Bonferroni correction.
