Figure 3
From: Lipid membrane templated misfolding and self-assembly of intrinsically disordered tau protein
Fluorescence microscopy (FM) images of DMPG lipid monolayers at 25 mN/m before (0) and 10 (1), 30 (2), and 60 (3) minutes after injecting hTau40 (a)42, K18 (b) or hTau40/3Epi (c) into the subphase. FM images of hTau40 (a0–a3) were reproduced from Jones et al.42. % trough area expansion due to tau membrane insertion at the time points are also indicated. Head group-labeled fluorescent dye TR-DHPE (0.5 mol%) was included in the lipid monolayer to provide fluorescence contrast. Dark regions of the monolayer correspond to dye-excluded liquid-condensed (LC) domains whereas light, or fluorescent region of the monolayer corresponds to disordered liquid-expanded (LE) phase of the monolayer where the TR-DHPE dye is mixed with the DMPG lipids.
