Table 4 Primers for multiplex amplification of 13 SNP markers.
From: Forensic validation of a panel of 12 SNPs for identification of Mongolian wolf and dog
Locus | Name* | Sequence (5′-3′) | Tm | Amplicon size (bp) |
|---|---|---|---|---|
rs22103787 | rs22103787F | CAAGGAGGGGCTCAACCTCA | 64.86 | 225 |
rs22103787R | AGAAGGGGTGTGTTGGTTTCTACA | 63.12 | Â | |
rs22835438 | rs22835438F | GGGATTGAACCCCTGACCTGAGT | 66.77 | 163 |
rs22835438R | TGAACCATGACCACCCCTCGT | 67.10 | Â | |
rs23249721 | rs23249721F | TGAAATGCTCTCCGGCTGAGGT | 67.58 | 170 |
rs23249721R | TCCATGCTAAATGTCCCAGGAG | 63.44 | Â | |
rs23608542 | rs23608542F | ACTTCTCCACGCGGAAACACTT | 64.44 | 143 |
rs23608542R | GAAATGGAAGGTGGCATTAGAACC | 63.99 | Â | |
rs23882488 | rs23882488F | GTCCTAGTCCTGGTCCATAGAAGAGC | 63.87 | 183 |
rs23882488R | AAGTCCGAGAAAAGTGGAGGATAGAT | 62.89 | Â | |
rs24163825 | rs24163825F | GCTAGTCCTATGCCCTGAAACTCAT | 63.13 | 161 |
rs24163825R | TCCTTATGAGCCTTAGTTTTGCTACCT | 62.86 | Â | |
rs24189603 | rs24189603F | GGAGCTCGGAGTGACTATACTGGAAT | 64.17 | 92 |
rs24189603R | CCTCGGATGCAGTCTGTTTCTCTG | 66.63 | Â | |
rs24198287 | rs24198287F | GTAGGCCAGTCTTCAGTGTGGTGA | 64.91 | 323 |
rs24198287R | GGCATGATTCCTGGTGGTTTTC | 64.85 | Â | |
rs24355642 | rs24355642F | CTCATAAAATCGGGAGCCTTGCTT | 65.66 | 105 |
rs24355642R | GCAGTATACACAGCAATCCAGCAGA | 64.75 | Â | |
rs24383001 | rs24383001F | TACGTCTGACGCCAAAACCCACT | 67.02 | 120 |
rs24383001R | CACCTGCACATTTGGGCCACT | 67.61 | Â | |
rs24863098 | rs24863098F | GTGAGCAGAGTTGCATGGCACA | 67.01 | 147 |
rs24863098R | GCTGCTGCCTCTCAAGGACGA | 67.54 | Â | |
rs9089629 | rs9089629F | TGGATTTGTTGCCTCTCCCCTAGT | 66.17 | 147 |
rs9089629R | CTACACTGTAAATGGGCAGCTACATCC | 65.00 | Â | |
rs9159232 | rs9159232F | TCCCACCTACATCTGCTTCTGTGT | 64.33 | 169 |
rs9159232R | GGGATGACTTGTCAAGGAACTGAGAC | 65.53 | Â |
