Table 5 Extension primers for genotyping of 13 SNP markers.

From: Forensic validation of a panel of 12 SNPs for identification of Mongolian wolf and dog

Name*

Sequences

Tm

Expected size (bp)

rs22103787SF-Y

(T)7-CCTCTCCTCTCCTTTAGTTCAACA

60.28

32

rs22835438SF-K

(T)27-GGCGTCATGGTATATCTGTAAATCAG

62.00

54

rs23249721SR-R

(T)13-CCCAGAATTTGAGATAACAAACATCA

62.44

40

rs23608542SR-Y

(T)17-AAAATGTTAGCGTAGAGCCGTTATT

60.69

43

rs23882488SF-K

(T)24-AAATAGCTCATTTGCCCAGATATG

63.13

49

rs24163825SR-M

(T)31-GCTACCTATTTGGTGAGAATAGTAAT

59.11

58

rs24189603SR-Y

(T)12-GTTTCTCTGTAGCCCTGTTTGTCAC

62.54

38

rs24198287SF-R

(T)17-CTTCATCAGTTACAAATAATTGTGGTG

60.10

45

rs24355642SR-R

(T)31-CAGTAACTTCCCTTCGTTTAATTAGTCTAC

60.52

62

rs24383001SR-R

(T)2-CTTGGAACCGAGAATGAAGAGC

62.46

25

rs24863098SF-M

(T)26-GGAGTGCATAATGATCCCTGTGTAC

62.94

52

rs9089629SF-R

(T)9-AACCACTGGATTACTGCCATTGA

62.82

33

rs9159232SR-Y

GCGTCCCTGTGACCTAGTTTC

61.06

22

  1. *F and R stand for forward and reverse primer; letters Y, K, R and M behind the short dash—are degenerate codes for nucleotide options at SNP sites: Y = C/T, K = G/T, R = A/G, M = A/C.
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