Figure 4

Uptake of SGC-7901, MKN-45, MKN-28 derived exosomes by immune cell subpopulation. The percentage fluorescent signal of exosome uptake by immune populations in the bone marrow BM, spleen, and lung was analyzed post 48 h by flow cytometry after injection of DiD-labeled SGC-7901, MKN-45, MKN-28-derived exosomes (20 μg/mice) or PBS (control) in C57BL/6 mice. (A) Representative flow cytometric plots (left) and quantification (right) of distinct DiD⁺ population within CD45⁺ cells. (B–F), Frequency of the DiD⁺ subpopulation within macrophages (Mø; CD11b⁺/F4/80⁺, B), DCs (CD11C⁺/MHCII⁺, C), CD8 T cells (CD3⁺/CD8⁺, D), NK cells (NK1.1⁺/CD3⁻, E) and CD4 T cells (CD3⁺/CD4⁺, F). Results are presented as mean ± SEM and analyzed by ANOVA, *, P < 0.05, **, P < 0.01, ***, P < 0.001.