Figure 3

Mce1C and Mce1D were localized in the cell wall. (a) The recombinant Mce1C or Mce1D protein from E. coli can be identified by anti-Mce1C or Mce1D sera, respectively. Lane 1 Mce1C protein. Lane 2 Mce1D protein. (b) Subcellular localization of Mce1C and Mce1D protein were analyzed using Western blotting with anti-Mce1C or Mce1D sera at a dilution of 1:4,000. Lane M Protein marker. Lane 1 whole cell lysate. Lane 2 cytosol and cell membrane. Lane 3 cell walls.