Table 1 Primers used for PCR amplification of mce1C or mce1D.
Gene | Primer | Sequence (5′ to 3′) | Vector |
|---|---|---|---|
mce1C | CF | GTATCATATGATGAACGAGCAGCGATCTCC | pET30a |
CR | GCTCAAGCTTTCGGGGCGGCTCCTGCACGG | ||
mce1D | DF | GTATCATATGATGAACAGCTCGAAGATCTT | |
DR | CTAACTCGAGTCGTCCACCTCCCGGAATCG | ||
mce1C | CF | TCAGGAATTCATGAACGAGCAGCGATCT | pMV261 |
CR | GATCAAGCTTTCATCGGGGCGGCTCCTGC | ||
mce1D | DF | TCAGGAATTCATGAACAGCTCGAAGATC | |
DR | GATCAAGCTTTCATCGTCCACCTCCCGG | ||
mce1C | CF | GCTCAAGCTTATGAACGAGCAGCGATCTCC | pcDNA6A |
CR | GTATGAATTCTCGGGGCGGCTCCTGCACGG | ||
mce1D | DF | GCTCAAGCTTATGAACAGCTCGAAGATCTT | |
DR | GTATGAATTCTCGTCCACCTCCCGGAATCG |
