Figure 2
From: UQCRH downregulation promotes Warburg effect in renal cell carcinoma cells
Restored UQCRH expression in KMRC2 repolarized mitochondrial membrane potential. (a) Inverse correlation of methylation and mRNA level of UQCRH in 21 ccRCC cell lines, data available from CCLE and drawn in Graphpad. (b) Methylation level of UQCRH and UQCRHL in 21 ccRCC cell lines, data available from CCLE and drawn in Graphpad. (c) UQCRH protein expression in 293T, 786O, RCC4 and KMRC2, detected by western blot. (d) Dose-dependent effect on UQCRH protein expression in KMRC2 by decitabine treatment, detected by western blot. (e) UQCRH ectopic overexpression in KMRC2 and UQCRH knockout by CRISPR/Cas9 in 786O, detected by western blot. (f) Principle and representative result of JC-1 assay with MFI of green fluorescence signals labeled. When mitochondrial membrane potential is disrupted, JC-1 cannot enter mitochondria effectively and remain as green fluorescent monomer in the cytosol.
