Figure 4

UQCRH overexpression reduces in vitro proliferation and in vivo tumor growth for KMRC2 cells. (a) Representative images of PI-stained nuclei and quantification result at the endpoint of the proliferation assay on the two KMRC2 sublines. Scale bar 300 μm. (b) Representative flow cytometry plots and quantification result of the apoptosis assay on two KMRC2 sublines. (c) Detection of cleaved caspase 3 in KMRC2-pcDNA and KMRC2-UQCRH by western blot. KMRC2-pcDNA treated with Staurosporine was used as positive control. (d) Tumor volumes in Rag1 knockout mice injected subcutaneously with KMRC2-pcDNA and KMRC2-UQCRH. (e) Representative images of H&E staining and IHC of UQCRH and cleaved caspase 3 in KMRC2-pcDNA and KMRC2-UQCRH tumors. Scale bar 100 μm. (f) IncuCyte confluency curves for 786O-scramble and 786O-sgRNA proliferation in vitro. (g) Representative images and quantification result of crystal violet-stained colonies formed by 786O-scramble and 786O-sgRNA (n ≥ 4). (h) Representative H&E images and percentage of tumor area relative to kidney area for orthotopic tumors formed by 786O-scramble and 786O-sgRNA (n = 2/group). Blue arrows point to the tumor area. Scale bar 1.5 mm (upper) and 100 μm (lower). In all plots, data represent mean ± SD. * P < 0.05, ** P < 0.01, **** P < 0.0001, ^ns P > 0.05, by Mann–Whitney test (for h) or unpaired t-test (for others).