Figure 2

Phylogeny of luminous beetles. (a) Maximum likelihood tree inferred from 531 single-copy orthologous genes and estimated divergent times with fossil calibrations (red nodes) with D. melanogaster as an outgroup. (b) Bayesian tree of Elateroidea families inferred from 13 mitogenomic protein-coding genes with Tribolium castaneum as an outgroup. Luminous taxa in (a) and (b) highlighted in cyan. (c) Genomic composition (coding sequence (CDS), five types TEs (DNA, LINEs, SINEs, LTR and Unknown) and Others (the rest of the whole genome except CDS and TEs) of five fireflies. (d), (e) Amino acid (AA) sequence identities of 3,125 single-copy orthologous genes (SCGs) among five fireflies (d: average; e: each SCG (each dot)). Notches in (d) indicate the median value. Color lines and dashed line in (e) indicate the smoother of a locally weighted regression, and slope ratio of 1, respectively.