Figure 3
From: Gfi1 upregulates c-Myc expression and promotes c-Myc-driven cell proliferation
The SNAG and C-terminal ZF domains, but not the DNA binding and transcriptional repression activities of Gfi1, are required for c-Myc upregulation. (A) Hela cells were transfected with c-Myc alone or together with Gfi1, the P2A (left panels) or N382S mutant (right panels). (B) Schematic diagrams of Gfi1 and the different truncation mutants. (C) Hela cells were transfected with c-Myc along with the different forms of Gfi1. The expression of the indicated proteins was examined by Western blot analysis. Full-length blots are presented in Supplementary Fig. 8. (D) The results shown in C were quantitated using ImageJ and normalized based on the expression levels of β-actin.
