Figure 4
From: Gfi1 upregulates c-Myc expression and promotes c-Myc-driven cell proliferation

Gfi1 upregulates MycER protein independent of Miz-1. (A) Hela cells were transiently transfected with MycER, MycER V394D without or with Gfi1 prior to immunoblotting for the indicated proteins. (B) BaF3/MycER/Gfi1 and BaF/MycER-V394D/Gfi1 cells were treated with or without Dox and 4-HT for 24 h. Nuclear extracts were prepared and examined for the indicated proteins by Western blot analysis. Full-length blots are presented in Supplementary Fig. 9.