Figure 6
From: KRAS K104 modification affects the KRASG12D-GEF interaction and mediates cell growth and motility
KRASG12DK104Q inhibits NPIPA2, DUSP1 and IL6 expression compared to KRASG12D and KRASG12D/K104A. (A) H1299 and MCAS cells were transfected with a KRASWT or KRASG12D, KRASG12D/K104A or KRASG12D/K104Q plasmid. We analyzed global gene expression profiles using human oligonucleotide DNA microarrays with three independent RNA samples at 48 h posttransfection. The intensity of each spot was analyzed by GenePix 4.1 software (Molecular Devices). (B) Three identified genes, NPIPA2, DUSP1 and IL6, were upregulated in the presence of KRASG12D and KRASG12D/K104A and downregulated in the presence of KRASG12D/K104Q compared with expression in the control group. (C,D) NPIPA2, DUSP1 and IL6 expression was detected by RT-PCR and after transfection of the mutant forms of KRAS. Data are the mean ± SD from three independent experiments. *P < 0.05 vs. untreated control; two-tailed Student’s t-test.
