Figure 3 | Scientific Reports

Figure 3

From: Functional characterization of Schistosoma mansoni fucosyltransferases in Nicotiana benthamiana plants

Figure 3

Synthesis of LeX on kappa-5 N-glycans by fucosyltransferases of S. mansoni (SmFucTs). Kappa-5 was co-expressed in ΔXT/FT N. benthamiana plants with sialDrGalT (GalT) and SmFucTs (A to F, H and J to L) or sialTnFucT9a as a positive control. After extraction of apoplast proteins the glycan composition was analyzed. (A) Aleuria aurantia lectin (AAL) binding assay for detection of α1,3-linked fucose. (B) LeX western blot to reveal the presence of LeX. (C–E) MALDI-TOF MS profiles of N-glycans released from purified kappa-5 upon co-expression of sialDrGalT with SmFucTD (C), SmFucTE (D) or SmFucTF (E).

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