Figure 4 | Scientific Reports

Figure 4

From: Functional characterization of Schistosoma mansoni fucosyltransferases in Nicotiana benthamiana plants

Figure 4

Fucosylation of LDN on kappa-5 N-glycans by fucosyltransferases of S. mansoni (SmFucTs). Kappa-5 was co-expressed in wild-type N. benthamiana plants with CeGalNAcT and SmFucTs (A to F, H and J to L) or sialTnFucT9a as positive control. After extraction of apoplast proteins the glycan composition was analyzed. (A) Soybean agglutinin (SBA) lectin binding assay for detection of GalNAc. (B) LDN-F western blot to reveal the presence of LDN-F. (C) F-LDN western blot to reveal the presence of F-LDN. (D–G) MALDI-TOF MS profiles of N-glycans released from purified kappa-5 upon co-expression of CeGalNAcT with sialTnFucT9a (D), SmFucTD (E) SmFucTE (F) SmFucTF (G). When a MS peak represents multiple N-glycan structures of identical mass, the possible positions of these sugar residues on the N-glycan are indicated between brackets.

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