Figure 4
From: Novel carbon film induces precocious calcium oscillation to promote neuronal cell maturation
Intracellular calcium oscillations in DIV 1 neurons after application of activity blockers: (a) representative traces of intracellular calcium fluctuations in the presence of TTX or the blocker cocktail; (b) relative number of active cells in DIV 1 cultures grown on nC substrate, control vs. TTX (individual values are plotted as open circles, mean value is plotted as the horizontal line, error bars represent SEM), the number of active cells in sister cultures grown on glass is set to 1 and marked with the dashed line (control: 7 independent cultures, n = 21 individual coverslips; TTX: 3 independent cultures, n = 7 individual coverslips, *p = 0.018, **p = 0.0065, one-sample t-test); (c) intervals between calcium peaks in DIV 1 neurons grown on nC or glass substrates and recorded in the presence of TTX (mean values, error bars represent SEM; 3 independent cultures; glass n = 292, nC n = 59; n/s: p > 0.9 when compared to same-substrate cultures recorded without TTX (Fig. 3d), two-way ANOVA with Bonferroni post-hoc test); (d) mean power spectral density curves of neuronal activity frequencies before and after the blockers application (3 independent cultures; glass + TTX n = 47, nC + TTX n = 40, glass + blockers n = 14, nC + blockers n = 46 cells recorded).
