Figure 2
From: Experimental adaptation of dengue virus 1 to Aedes albopictus mosquitoes by in vivo selection
Experimental design for DENV-1 adaptation to Ae. albopictus. The parental strains 1806 (France) and 30A (Thailand) were passaged 10 times on a single Ae. albopictus population from Nice, France. Each passage includes: mosquito infectious blood-meal with DENV-1, collection of mosquito saliva at day 21 post-infection, viral amplification of saliva on Ae. albopictus cell cultures for 8 days, and initiation of the next passage using the viral suspension obtained. Control isolates were serially passaged 10 times on C6/36 cells. Two replicates R1 and R2 were performed.
