Figure 4
From: Experimental adaptation of dengue virus 1 to Aedes albopictus mosquitoes by in vivo selection
Infection, Dissemination and Transmission of DENV-1 (Parental, P10_R1, and P10_R2) by Ae. aegypti Pazar and Ae. albopictus Nice. Mosquitoes were exposed to blood meals at a titer of 107 FFU/mL. Females were examined at 7, 14 and 21 dpi. Mosquito body (thorax and abdomen) and head were processed individually to determine (a,b) the infection rate (IR, proportion of mosquitoes with infected body among the engorged mosquitoes) and (c,d) the dissemination efficiency (DE, proportion of mosquitoes with infected head among tested mosquitoes). (e,f) Saliva was collected from individual females to determine the transmission efficiency (TE, proportion of mosquitoes with infectious saliva among tested mosquitoes). The Parental strain corresponds to DENV-1 1806, and P10_R1 and P10_R2 refer, respectively, to replicate 1 and replicate 2 of the 10th in vivo passages of DENV-1 1806 on Ae. albopictus. Asterisks refer to a significant difference (***p < 10−3). In brackets, the number of mosquitoes tested. The error bars correspond to the confidence intervals (95%).
