Figure 6
From: Experimental adaptation of dengue virus 1 to Aedes albopictus mosquitoes by in vivo selection
Analysis of mutation frequencies in the DENV-1 3′UTR. (a) Schematic overview of the DENV-1 3′UTR secondary RNA structure, indicating from 5′ to 3′ the stem loop (SL)-I, SL-II, dumbbell (DB)-1, DB-2 and 3′SL RNA structures. Single nucleotide variants with a frequency ≥ 0.05 after 10 passages of DENV-1 1806 or 30A in either C6/36 or mosquitoes are highlighted in red. Pseudoknots and other tertiary RNA interactions are indicated by the black lines. (b) Analysis of the mutation frequencies in SL-II and the 3′SL of the parental DENV-1 30A and 1806 sequences, and passages P10 (1806) and P10 (30A). The mutation frequency is indicated by color on a scale from 0 (white) to 1 (red). The figure was prepared using the free software VARNA RNA editing package version 3-93. (https://varna.lri.fr/index.php?lang=en&page=downloads&css=varna) and modified with Adobe Illustrator CC.
