Figure 8
From: Experimental adaptation of dengue virus 1 to Aedes albopictus mosquitoes by in vivo selection
Reverse genetic constructs with the 10,418 mutation do not show higher transmission in Ae. albopictus. (a) Transmission of reverse genetic constructs (Parental construct, P10 construct 1, P10 construct 2) by Ae. albopictus Nice with reference to Parental and P10 strains. Twenty-one days after an infectious blood meal at a titer of 107 FFU/mL, mosquitoes were processed for saliva collection to determine the transmission efficiency (TE, proportion of mosquitoes with infectious saliva among tested mosquitoes). (b) Viral loads in saliva were estimated by focus fluorescent assay on Ae. albopictus C6/36 cells. (c,d) A second replicate using the P10 construct 1 was performed. Bars indicate the mean.
