Table 1 Two-way ANOVAs for the effects of CO2 and N-fertilizer levels, and their interaction on the belowground and aboveground biomass, foliar contents of total soluble protein and Bt toxin, Bt-transgene expression and methylation in promoter and coding regions of Bt rice with fused Cry1Ab/Ac during tillering stage, grown under ambient and elevated CO2 with different N-fertilizer levels (F and P values).

From: Elevated CO2 alters transgene methylation not only in promoterregion but also in codingregion of Bt rice under different N-fertilizer levels

Parameters

CO2 level (CO2)

N-fertilizer level (N)

CO2 × N

F-values

P-values

F-values

P-values

F-values

P-values

Aboveground biomass (g; fw)

9.30

0.003

193.81

< 0.001

3.29

0.04

Belowground biomass (g; fw)

12.24

< 0.001

244.28

< 0.001

3.25

0.04

Foliar total soluble protein (mg/g; fw)

1.43

0.24

15.73

< 0.001

2.02

0.15

Foliar Bt protein content (μg/g; fw)

0.58

0.46

72.99

< 0.001

3.54

0.045

Bt gene expression

3.84

0.07

4.55

0.03

16.61

< 0.001

Promoterregion methylation of P1 (%)

22.27

< 0.001

4.00

0.047*

23.54

< 0.001

Codingregion methylation of P2 (%)

1.61

0.23

0.05

0.95

3.02

0.086

Codingregion methylation of P3 (%)

0.004

0.95

0.28

0.76

0.46

0.64

Codingregion methylation of P2 + P3 (%)

1.70

0.22

0.13

0.88

3.92

0.049

Transgene methylation of P1 + P2 + P3 (%)

19.82

< 0.001

1.84

0.20

13.34

< 0.001

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