Figure 2 | Scientific Reports

Figure 2

From: Lineage tracing reveals evidence of a popliteal lymphatic muscle progenitor cell that is distinct from skeletal and vascular muscle progenitors

Figure 2

PLV-LMCs do not derive from skeletal muscle progenitor cells during development. Multicolor fluorescent microscopy was performed on whole mount immunostained PLVs from constitutive Pax7Cre (n = 7, P200 depicted) and MyoDiCre (n = 3, P21 depicted) reporter mice as described in Fig. 1, and direct fluorescent microscopy was performed on Hoechst stained diaphragm tissue from the same muscle-reporter transgenic mice as a positive control. Representative low-magnification images of PLVs with a highlighted region of interest (white boxes) (A.a,B.a) are shown with corresponding high-magnification images of Prox1 immunostain of LECs (A.b,B.b), αSMA immunostain of LMCs (A.c,B.c), the Cre-driven tdT expression (note lack of red fluorescence within the white dotted lines that outline the PLV) (A.d,B.d), and overlay of all channels (A.e,B.e). Representative images of diaphragm tissue are shown to illustrate the robust Cre-driven tdT expression in skeletal myocytes from these mice (A.f,B.f).

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