Figure 2 | Scientific Reports

Figure 2

From: Mechanoadaptive organization of stress fiber subtypes in epithelial cells under cyclic stretches and stretch release

Figure 2

Microscopic images and quantitative analysis of A549 cells in response to cyclic stretches. (A) Representative microscopic images of the cell body (phase contrast) and nucleus (Hoechst) after uniaxial cyclic stretch (CS) at 5%, 10%, and 15% strains for the indicated time points. The time kinetic reorientation of cells in the presence of CS and post-CS release is shown in the left and right panels, respectively. Scale bars: 100 µm. (B) Kinetic profiles of the reorientation angle, elongation parameter, of the cell bodies in cells subjected to CS and post-CS release (n = 200). (C) Kinetic profiles of the reorientation angle, elongation parameter, of the cell nuclei in cells subjected to CS and post-CS release (n = 200). Reorientation data are presented in the box–whisker plot corresponds to 5 and 95 percentiles. Elongation and area data are presented as mean ± SD. ns = not significant, *p < 0.01; one-way analysis of variance (ANOVA).

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