Table 9 Verification conditions and results (n = 3).

From: Optimization of membrane dispersion ethanol precipitation process with a set of temperature control improved equipment

CPPs and CQAs

V1

V2

V3

Concentrates

N11

N13

N11

Is it in the design space?

Yes

Yes

No

 DM (%)

45

45

45

 ECR (g/g)

1.9

1.9

1.2

The ethanol solution concentration (%)

93

94

90

 Temperature (°C)

5

5

5

 Dry matter removal (%)

  Experimental value*

49.23 ± 0.64

50.95 ± 2.46

37.88 ± 1.20

  Predicted value

49.82

51.14

38.71

 Purity of Astragaloside IV (μg/g)

  Experimental value*

1007.19 ± 60.07

899.73 ± 73.14

1083.41 ± 50.90

  Predicted value

1119.77

865.77

1119.77

 Purity of astragaloside II (μg/g)

  Experimental value*

948.76 ± 45.37

984.53 ± 28.71

893.08 ± 20.73

  Predicted value

911.93

867.57

821.78

 Purity of CG (μg/g)

  Experimental value*

2691.32 ± 133.66

2733.53 ± 97.48

2451.62 ± 24.05

  Predicted value

2471.65

2539.55

2350.18

 Purity of PG (μg/g)

  Experimental value*

1054.72 ± 46.97

765.27 ± 25.26

939.22 ± 25.75

  Predicted value

1044.77

930.65

1074.02

 Purity of IFG (μg/g)

  Experimental value*

887.45 ± 82.71

692.58 ± 23.22

869.80 ± 19.20

  Predicted value

783.24

625.17

765.00

  1. Experimental value*: average value ± SD.