Figure 2

Effect of PEG concentration on size and density of A_2AR crystals grown in the EROCOC₁₇₊₄-matrix. (A) Size (black) and density (magenta) of EROCOC₁₇₊₄-matrix grown A_2AR crystals as a function of the PEG400 concentration, in an otherwise fixed solution composed of 50 mM NaSCN, 2% 2,5-hexanediol, and 100 mM Na citrate, pH 5.50. (B) Cross polarized (upper) and second harmonic generation (lower) images in a 96-well glass screening plate, showing the birefringence and chirality-derived signals of the protein crystals, respectively. (C) Crystals used for SS-ROX under optimized conditions: 38% PEG400, 50 mM NaSCN, 2% 2,5-hexanediol, and 100 mM Na citrate, pH 5.75. Upper: cross polarized, lower: bright field.