Figure 3
Comparison of EROCOC17+4- and MO-matrix grown A2AR crystal structures. (A) Crystal structures of LCP-SFX_4 °C (magenta) and 4EIY (cyan). The bRIL moiety inserted in ICL3 is omitted from the coordinates (magenta dashes). Sodium ions (green and dark grey balls for LCP-SFX_4 °C and 4EIY, respectively) and ZM241385 (red and dark grey sticks for LCP-SFX_4 °C and 4EIY, respectively) are also shown. Black lines approximately correspond to the hydrophobic/hydrophilic boundary of the lipid bilayer. IC and EC are intracellular and extracellular sides, respectively. Two well characterized EROCOC17+4 (red; E1 and E2) and three cholesterol (orange; C1, C2 and C3) models are displayed as sticks. (B,C) Molecular surface of LCP-SFX_4 °C with well characterized lipids. The 2mFo-DFc electron density maps around the lipid models are contoured at 1.0 σ (pink mesh). The carbon atoms of the EROCOC17+4 and cholesterol stick models are shown in magenta and orange, respectively. The oxygen atoms of both lipids are shown in red. This figure is prepared by PyMOL Ver. 1.8 (https://pymol.org).
